|
KMID : 0624620130460060328
|
|
BMB Reports
2013 Volume.46 No. 6 p.328 ~ p.333
|
|
|
Reconstruction of radial bone defect using gelatin sponge and a BMP-2 combination graft
|
|
Kim Seong-Gon
Jeong Jae-Hwan
Che Xiangguo
Park Yong-Tae
Lee Sang-Woon
Jung Eun-Sun
Choe Se-Nyon
Choi Je-Yong
|
|
|
Abstract
|
|
|
|
Many bioactive molecules like recombinant human bone morphogenetic protein 2 (rhBMP-2) have been developed for mineralized bone grafts, for which proper scaffolds are necessary to successfully apply the bioactive molecules. In this study, we tested the osteogenic efficacy of rhBMP-2 produced in-house in combination with gelatin sponge as the scaffold carrier in a rabbit radial defect model. The efficacy of the rhBMP-2 was determined by alkaline phosphatase activity assay of C2C12 cells. Two groups of ten rabbits each were treated with rhBMP-2/gelatin sponge, or gelatin sponge only. At 4 weeks, rhBMP-2/gelatin sponge grafts showed more bone regeneration than gelatin sponge grafts, as determined by X-ray radiography, micro-computed tomography, and histological analyses. At 8 weeks, rhBMP-2/gelatin sponge grafts exerted much stronger osteogenic effects. The study demonstrates the improved osteogenic efficacy of the rhBMP-2/gelatin sponge grafts in a rabbit radial bone defect model acting as a bone-inductive material.
|
|
|
KEYWORD
|
|
|
BMP-2, Bone defect, Bone graft, Gelatin sponge, Osteogenic activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|